SFB 1243 Cancer Evolution

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qPCR Seminar

Best practices for quantitative PCR


Registration is open!

Date and Time: February 19, 2020,  from 13:15-16:00

Location: BMC, room N02.017

Trainer: Dr. Jan Winter, Application Specialist at Steinbrenner Lab Systems

Dr. Jan Winter will offer sessions for both beginners and experienced users: qPCR basics, followed by  an advanced session with troubleshooting tips. Participants can state their own qPCR questions and/or challenges on the registration sheet.

Open to users of all levels – students, doctoral researchers, postdocs, technicians.

Participation is free of charge. Space is limited –registration required! https://seminar.qpcr-guide.com

Download invitation/description (pdf, 3 MB)

Best practices for quantitative PCR, program

13:15-14:15 Session 1: qPCR Basics and Optimal Assay Design

  • Background Knowledge
  • Applications
  • ASsay Design
  • TaqMan or Dye?
  • Primer Design
  • Multiplexing
  • Assay Validation

Coffee break

14:45-15:45 Session 2: Advanced Topics and Troubleshooting

  • Data Analysis and Software
  • Pitfalls
  • Common mistakes
  • Strategies for Assay Optimization
  • Troubleshooting of your specific problems