Comparative Analysis of Single-Cell RNA Sequencing Methods
Ziegenhain et al. (2017) Molecular Cell 65, 631-643
17.02.2017
Authors (SFB 1243 members): Christoph Ziegenhain, Beate Vieth, Swati Parekh, Björn Reinius, Amy Guillaumet-Adkins, Martha Smets, Heinrich Leonhardt, Holger Heyn, Ines Hellmann and Wolfgang Enard
DOI: http://dx.doi.org/10.1016/j.molcel.2017.01.023
LMU press release (German)
Cited from "In Brief" and "Highlights" in the article:
Ziegenhain et al. generated data from mouse ESCs to systematically evaluate six prominent scRNA-seq methods. They
used power simulations to compare cost efficiencies, allowing for informed choice among existing protocols and providing a framework for future comparisons.
The study
- represents the most comprehensive comparison of scRNA-seq protocols,
- provides power simulations quantifying the effect of sensitivity and precision on cost efficiency,
- offers an informed choice among six prominent scRNA-seq methods,
- provides a framework for benchmarking future protocol improvements.